The Ospreys await their Heineken Cup fate after Leicester Tigers made a complaint as the Ospreys played with 16 men for part of their 17-12 triumph.
The Welsh region knocked Leicester out as they qualified for the quarter-finals with their Liberty Stadium win.
The confusion was in the final quarter when full-back Lee Byrne returned from the blood-bin and his replacement Sonny Parker stayed on for a brief period.
The Ospreys are likely to be hit with a fine rather than European expulsion.
Tournament organisers European Rugby Cup will investigate the events in Swansea after Tigers chief executive Peter Wheeler and chairman Peter Tom lodged an official complaint immediately after the dramatic showdown.
Competition rules suggest the match result must stand, yet the Ospreys still face an anxious wait to discover what punishment – if any – ERC decides to impose.
England were fined £10,000 during the 2003 World Cup when wing Dan Luger featured as a 16th player for 34 seconds of their group-stage win against Samoa in Melbourne.
Unlike in Luger’s case, it is claimed on this occasion that Ospreys’ Wales and British Lions full-back Lee Byrne was involved in helping to thwart an opposition attack.
Byrne had gone off suffering from a dislocated and bloodied toe, being replaced by centre Sonny Parker, but Byrne was sent back on three minutes later without any of his team-mates going off.
Fly-half Dan Biggar’s departed in the 73rd minute after about a minute returned the Welsh region to 15 men as the Ospreys held on during a torrid finale of constant Leicester pressure.
A Leicester player pointed out the Ospreys’ numerical advantage to referee Alan Lewis, who then berated an Ospreys touchline official for not informing the match fourth official that Byrne had returned.
Lewis should have awarded Leicester a penalty once the extra-man situation became apparent.
Leicester chief executive Peter Wheeler said: “During a significant part of the game, the Ospreys had 16 players on the pitch, and he was involved in a break by Ben Youngs being stopped from developing.
“We think the interference of the 16th man was significant – you are only entitled to have 15 players on the pitch.
“We think it affected the outcome of the game. The players put their heart and soul into it out there, and they deserve to be treated fairly.”
Tigers chairman Peter Tom added: “A serious error has been made. We are 12 years or so into the professional game, and it is time administrative procedures were sorted out.
“There has been a significant mistake that could be said to have had an impact on the game.
“People who administer the game have to make sure it doesn’t happen again.”
Referee Alan Lewis gave a dressing down to an Ospreys official over the incident, telling her she should have told the game’s fourth official Byrne was returning to the field.
Byrne could be seen signalling to a team-mate he should depart, but did not leave the field when his message did not get through.
International Rugby Board rules state: “At any time before or during a match a team may make an objection to the referee about the number of players in their opponents’ team.
“As soon as the referee knows that a team has too many players, the referee must order the captain of that team to reduce the number appropriately.
“The score at the time of the objection remains unaltered.
“Sanction: Penalty at the place where the game would restart.”
No penalty was awarded by Lewis and the IRB regulation suggests the result will stand. However, ERC officials are duty-bound to investigate.
Leicester coach Richard Cockerill said: “I am sure they (Ospreys) didn’t do it purposely. I don’t think it had a bearing on the result.
“We were beaten fair and square. I am a lover of the game, and I am sure it was a mistake by somebody.”
Ospreys boss Scott Johnson said: “This is one for the officials. They can work it out.
“There was a call for Lee Byrne to come on and ‘X’ to come off – that was the instruction.
“Byrne came off as a blood bin, and maybe that’s where the confusion was. You just give instructions as a coach.”
And in a brief statement, the Ospreys said: “We are aware that a complaint has been lodged with ERC by Leicester Tigers.
“The matter is now in the hands of the ERC, and as such, it would be inappropriate for us to make any further comment on this issue.”
An ERC spokeswoman confirmed to BBC Sport that the ERC are “looking at the case” and will make a statement on Sunday.


